The owner of Aberdeen zero-waste outlet Refillosophy fears rising rates bill could leave her facing closure.
Gina Adie has branded the business rates system "archaic" and "not fit for purpose".
It comes after the draft business rates valuation roll was published, which shocked a number of Aberdeen businesses with huge potential increases in their rates bills come April, including Aberdeen Football Club's Pittodrie Stadium.
The Press and Journal says that, under the new proposals, Ms Adie is now liable for £9,337 from April.
Previously Ms Adie's store on Albyn Grove - which employs five part-time workers - came under the small business rate relief threshold so faced no rates payment obligations.
However, under the draft assessment her proposed rateable value is now £18,750 - above the £18,000 threshold for 25% relief.
'Furious'
Ms Adie said: "I'm extremely disappointed with our new rateable value - in fact I'm furious about it.
"We simply hadn't factored such a high additional cost into our business budgeting.
"What are our options: putting up our prices, laying off staff or - the worst-case scenario - closing a shop we've worked so hard to establish?
"I find it shocking and extremely frustrating when we keep hearing we want local shops and the city centre to flourish, yet it seems to be increasingly difficult for this to happen.
"The rates system is archaic. It's not fit-for-purpose due to the changing business landscape. With online shopping, we are competing in a difficult world.
"We should be encouraging smaller local firms into the city centre. Overnight we're now facing a bill of more than £9,000."
Plastics-free shopping
Ms Adie opened Refillosophy, based in Albyn Grove, just over two years ago and provides plastics-free shopping, local produce, recycled products and the re-use of containers where possible.
Customers can pick their coffee beans, loose tea, bakery products and fresh fruit and vegetables and other produce from the shelves.
The "shocking" adjustment in ratable value of her premises has left Ms Adie questioning what the future may hold for her business.
She said: "Someone said I should move to somewhere like Westhill where we may pay less rates.
"I walk to work as do my staff. A move out of town would involve car travel and that goes against our ethos.
"The whole point is that we are an eco-friendly business with sustainability at our core.
Forward thinking?
"Perhaps this should be considered in a forward-thinking rates assessment - one that looks into what we are trying to do.
"I felt we were getting back on our feet after the Covid-19 pandemic but now this. I'm not sure what to do to be honest."
The final rates bill will be dependent on any changes to the rates poundage in the Scottish Budget on November 15.
Ms Adie said she was considering an appeal.
Aberdeen & Grampian Chamber of Commerce said last week that the regeneration of Aberdeen city centre is facing a significant hurdle after several hospitality firms were dealt a business rates blow by the region's assessors.
Draft revaluation notices due to take effect on April 1 will see rateable values plummet for many in the north-east, with out-of-town retail among the big winners.
However, there was bad news for some businesses across the city centre, with many hospitality businesses in and around Union Street facing static or increasing bills.
Revolucion de Cuba, based in The Academy, could see a massive hike of 222% in its rateable value, which is rising from £60,000 to £193,000.
One of the biggest surprises was Aberdeen FC's Pittodrie Stadium, which will see its rateable value soar from £192,500 to £450,000 under the draft assessment.