Donald Trump has threatened to take out Iran "in one night" if it does not agree to a deal to fully reopen the Strait of Hormuz by midnight tonight.
The US president has made repeated threats and issued deadlines throughout the conflict, but rarely as specific as this one.
Should Iran not comply with the deal by 8pm Washington time, Trump has vowed to wipe out every bridge and powerplant.
Warning that "very little is off-limits", Trump said part of the deal will include "free traffic of oil" through the Strait.
The president added: "We'll see what happens. I believe they're negotiating in good faith. I guess we'll find out."
The Guardian reports Donald Trump has said he is “not at all” concerned about committing possible war crimes, adding: “You know what’s a war crime? Having a nuclear weapon."
Human Rights Activists news agency (HRANA) says nearly 3,600 people have been killed in the US-Israeli strikes on Iran since attacks began, including at least 1,665 civilians.
Israel has warned Iranians not to use the country's trains as it threatens to wipe out the entire network.
Global oil prices rose on Tuesday afternoon in Asia as the deadline approaches, the BBC reports, with benchmark Brent crude up by about 1.4% to $111.33 (£84.13) a barrel, while US-traded oil gained 2.8% to $115.61.
While Trump pushes for the free traffic of oil through the Strait of Hormuz, some Asian nations reliant on energy from the Gulf have already struck deals with Iran to allow some vessels through.
Nations including Pakistan, India and the Philippines have all made agreements with Tehran to let some ships pass through the strait safely, the BBC reports.
China has also acknowledged that their vessels have also used the channel.
Around 20% of the world's oil and gas shipments ordinarily travel through the Strait of Hormuz.