The Union Street branch of the Royal Bank of Scotland is set to close as the company announces more than 100 job losses.
The New Deer branch is also one of 18 local branches to close, more than a fifth of the national total.
The bank, owned by NatWest, confirmed 105 jobs would be impacted.
Royal Bank of Scotland said jobs will be lost on a voluntary basis, but will otherwise support any staff who lose their jobs.
A spokesperson confirmed no other branches would be closed until at least 2026.
Online banking surging ahead
The spokesperson said: "Our customers are using digital banking more than ever before – over 80% of our active current account holders now use our digital services and over 97% of retail accounts with us are now opened online.
"While we are increasingly engaging our customers digitally, our branch network remains important to us. We commit to no further review of our Royal Bank of Scotland branch network until at least 2026."
The branch in Aberdeen is set to close on October 9, while New Deer will lose its bank on September 26.
They added: "But we know that a small number of people are not yet comfortable with it, which is why we are proactively reaching out to support them with this transition, having made over 200,000 calls last year. We also have experts that they can speak to for support and guidance."